LOCUS       sequence-123291-         867 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.1, nuclear 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pNuc-rxRFP1.1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             129..161
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(165..173,174..188)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgccttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttgccg ccaccatggg aggttctcat catcatcatc
      121 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg
      181 acgataagga tccgagctcg agcgagctta gttgcgctat aggtcggctg agctcacccg
      241 tggtttccga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaaggggc
      301 tgaggctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
      361 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
      421 acgaggacta caccatcgtg gaacagtgcg aacgcgccga gggccgccac tccaccggcg
      481 gcatggacga gctgtacaag ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg
      541 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      601 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga
      661 ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      721 ctcagttcat gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact
      781 tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg
      841 gcattattca cgttaaccag gactcct
//
