LOCUS       sequence-12355-e        9677 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4AD
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1130..1150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1166..1507
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1513..1532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1513..1531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1538..1547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1550..1576
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2055..2088)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2273..2291)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2743..2814,3216..3489,3564..4218,4280..4595,4799..4930,
                     5001..5615)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(6389..6422)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6446..6479)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(6709..6910,6911..6941)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7478..7497)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7597..7619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7657..7675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7743..7847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7848..7916,7917..8708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8066..8085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8879..9467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9368..9387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccgcgg ccgcaaaaca tggataaagc ggaattaatt cccgagcctc caaaaaagaa
     1141 gagaaaggtc gaattgggta ccgccgccaa ttttaatcaa agtgggaata ttgctgatag
     1201 ctcattgtcc ttcactttca ctaacagtag caacggtccg aacctcataa caactcaaac
     1261 aaattctcaa gcgctttcac aaccaattgc ctcctctaac gttcatgata acttcatgaa
     1321 taatgaaatc acggctagta aaattgatga tggtaataat tcaaaaccac tgtcacctgg
     1381 ttggacggac caaactgcgt ataacgcgtt tggaatcact acagggatgt ttaataccac
     1441 tacaatggat gatgtatata actatctatt cgatgatgaa gataccccac caaacccaaa
     1501 aaaagagatc tttaatacga ctcactatag ggcgagcgcc gccatggagt acccatacga
     1561 cgtaccagat tacgctggag gaggtggtgg aggtggaggt ggaggtacta gtctggagat
     1621 cgaggccgcc ttcctggagc gcgagaacac cgccctggag acccgcgtgg ccgagctgcg
     1681 ccagcgcgtg cagcgcctgc gcaaccgcgt gagccagaac cgcacccgct acggccccct
     1741 gggcggcggc aagtaaggcg cgcctctaga tcgataccgt cgactaaagc caaatagaaa
     1801 attattcagt tcctggctta agtttttaaa agtgatatta tttatttggt tgtaaccaac
     1861 caaaagaatg taaataacta atacataatt atgttagttt taagttagca acaaattgat
     1921 tttagctata ttagctactt ggttaataaa tagaatatat ttatttaaag ataattgcgt
     1981 ttttattgtc agggagtgag tttgcttaaa aactcgttta gatccactag cttaattaag
     2041 catgcctagg ctagataact tcgtatagca tacattatac gaagttatag cttctgatgg
     2101 aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga ggctgctgat
     2161 ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag aaccacaaag
     2221 tgggaatcaa gagaaaaaca atgatcccac gagagatcga attctagtat gtatgtaagt
     2281 taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact
     2341 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg
     2401 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     2461 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     2521 ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc
     2581 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     2641 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     2701 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     2761 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     2821 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     2881 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa
     2941 atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag
     3001 atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa
     3061 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     3121 aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc
     3181 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
     3241 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc
     3301 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
     3361 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
     3421 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
     3481 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
     3541 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     3601 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     3661 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     3721 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     3781 aacggccagg gaacacctga ttttccaagc catggtgcgg atgccacgac atctgaccta
     3841 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     3901 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     3961 tctggcattc gcctccgagg ctctaaccga tccgccgctt ctgatctgcg atgagcccac
     4021 ctccggactg gactccttta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     4081 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
     4141 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     4201 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag tattacataa
     4261 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
     4321 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
     4381 ccaagatatg cgacaatttt gccattagca aagtagcccg ggatatggag cagttgttgg
     4441 ccaccaaaaa tctggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca
     4501 cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac
     4561 cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat
     4621 gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagataga attgcctgat
     4681 tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaagataa
     4741 ataaatttca tttaactcgc gaatattaat gagatgcgag taacatttta atttgcagat
     4801 ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat
     4861 gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc
     4921 cacgataaat gtaagtcatg tttagaatac atttgcattt caataattta ctaactttct
     4981 aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc
     5041 gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattgccgc
     5101 tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg
     5161 ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa
     5221 cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg
     5281 gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg
     5341 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
     5401 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
     5461 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
     5521 tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
     5581 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
     5641 tgtttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
     5701 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
     5761 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
     5821 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
     5881 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
     5941 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
     6001 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
     6061 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
     6121 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
     6181 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
     6241 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
     6301 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
     6361 tgcttggatt tcactggaac tagaattcat aacttcgtat agcatacatt atacgaagtt
     6421 atggatctgg ccgcgctctt ccgctgaagt tcctatactt tctagagaat aggaacttcc
     6481 tcgacggtag cggtccggtt gttttcgtgc tcatcgcgag tacgtattta tttttaaaaa
     6541 ataataaacg cggcaaacac aatcacacaa atgtgcaggc acagtgggac aaagtgcgct
     6601 aaatttggat atgtaataaa tccaaaacca aaaagaaaac aaaaaacggt aaaatatttt
     6661 actgttgtta aaattcgatc attcattatt cgctgcatga attagctttg cgtactcgca
     6721 aattattaaa aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca
     6781 aaccccacgg acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa
     6841 tacgacactc agaatactat tcctttcact cgcacttatt gcaagcatac gttaagtgga
     6901 tgtctcttgc cgacgggacc accttatgtt atttcatcat ggtctggcca ttctcatcgt
     6961 gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc
     7021 gacatatatc cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact
     7081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     7141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     7201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     7261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     7321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     7381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     7441 ggtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     7501 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7561 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7621 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9001 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9241 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     9301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct
     9481 tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt
     9541 acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct
     9601 gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact
     9661 tctccgccgc cgatctg
//
