LOCUS       sequence-12361-e       10400 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-QF
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3820..3853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4038..4056)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4508..4579,4981..5254,5329..5983,6045..6360,6564..6695,
                     6766..7380)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(8154..8187)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8212..8232)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8212..8230)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8251..8267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8251..8267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8264..8286)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8275..8291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8299..8305,8306..8323,8324..8329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8344..8365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8482..8499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8653..9241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8733..8752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9412..10203,10204..10272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10035..10054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10273..10377)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
      721 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
      781 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
      841 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
      901 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg aggtcgacgg
      961 tatcgataag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
     1021 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg atccaaaatg
     1081 ccgcctaaac gcaagacact caatgccgct gccgaagcca atgcccacgc tgatggccat
     1141 gctgatggca atgctgatgg tcatgtcgct aacactgcag caagcagcaa caacgcccgt
     1201 tttgcggact tgaccaacat tgacacaccc ggcctcggcc ctaccacgac gacgttactt
     1261 gtcgagcccg ctcgttcgaa acgccagaga gtctcgaggg cctgtgatca gtgtcgagct
     1321 gcacgtgaaa agtgtgatgg aatccagccg gcttgcttcc cctgtgtgtc gcagggccgg
     1381 tcgtgtacct accaggccag tcccaagaag cgaggagtcc agacgggcta catccgcact
     1441 ctcgaactgg ctctggcttg gatgttcgag aacgttgccc gcagcgagga cgccctccac
     1501 aatcttttgg tccgtgatgc tggccagggc agcgctctcc tggtcggcaa agactcgcct
     1561 gctgcagaac gcctgcatgc aagatgggcg acgagtcgag tcaacaaaag catcacccgt
     1621 cttctctcag gtcaggccgc acaagatcca tctgaagacg gccaatcccc gtccgaagac
     1681 ataaatgtcc aagatgccgg ggcaaagaca tccgacttcc ctcatgcgcc tcacttgact
     1741 ttctcggcgc ctaagtctag tacagctgag acacgcactc taccaggccc ggtccgacca
     1801 cctatttcgg caaacaccct ggaaaacaac cttcagccag atggtaccgg gatagggaag
     1861 ctaccaccca atcattggcg cctgctggat atctactttt cctacacgca ttcttggctc
     1921 cctatcctcg agaagaaaga catgtaccaa gcattgtacc agtactctga acaaggctcg
     1981 ttgcttccct ctgcgaatgt cgagtctggc gttcatgccg agctctggag cgcgctcgcc
     2041 ctggcgtcct tccaggctgc tgctactgct gcatcgagtg ctacgggtcc agcttcagct
     2101 gctcatggcc atgacaatgc catcaatcct tcacctgcag acatatctga cacagcccga
     2161 aagctcatac ctttggaaag cgggccgttc caggttcagc actgcagagc gttgctgctt
     2221 ctttgtctcg taagccttgg gcgggatgat tgggagtctg cttggttgct ggttggcttt
     2281 gcggtccgcg tcctacttgt tgttcgcacc cagttgcctc ctgatgatga ccggccacga
     2341 ccaagaatgc gtgcgctgct cgtcgcgtgc ttcatcgtgg ataccattgt gtctatgaga
     2401 cacaacgtgc cggcccatct caagccagac gacattgcgg atctgccgtt acctgaagac
     2461 ggtcaagatc aatgggagcc gtggacacca tgtgagggct taggcggtga acacaccatg
     2521 ctgcaaatgt tgaggaaccc ggcataccct ttaagcacat tcaaccacct atatggcgtg
     2581 accaagctgg ttgctttgga gcttctgcca agaatacgaa catcttcaca gaacgctccc
     2641 ttggagttca ggtcgcggtt gcagcaggta atcggccaca attctccctt cagcgtcttt
     2701 gtcctttccc aggatacagc atcggctttt gtgcctactg cataccttac ccgtaccgtt
     2761 tatttatggg cagctgcctt ttctgagcct ctcaacgaac actactcgca tcttctgatc
     2821 gaaactcttg atcagtatca gaagcggttt ggtacatatg caatcccacc tctgatccct
     2881 tctcttctag actcccttct tgctttaaag aaacaatcac attcttcaga gcggcatcga
     2941 aggcacttgg aagagctttt ccccgcctac tcctccattt ggcctcgggg aggccgacac
     3001 agcaatactg gcctccaacc catacgacaa cttgagcttc cgccgactgc gactgccact
     3061 gcaagtatca tgccccatgt catggaacag cccctgtcaa cgtcaataaa tccggtcaat
     3121 gatcggttta atggaatacc gaatcctacc ccctacaata gcgatgcggc cctagacgca
     3181 attactcaaa ccaatgacta cggatcagtc aatacccatg gcattctcag tacatatcca
     3241 ccacctgcca cccacttgaa cgaggcctcg gtggcccttg cgcctggagg tgctcctccc
     3301 agaccacccc cgccatacgt tgatagtaca acgaaccatc ctccttacca tagtaacctc
     3361 gtccccatgg ccaatttcgg atattcgact gtagactatg atgcgatggt ggatgatctg
     3421 gcatcgatcg agtacacgga tgcggttgac gttgatccgc agttcatgac gaacctcggg
     3481 tttgtcccag ggtgtaattt tagcgatatc aacacgtatg agcaatagta agcactagat
     3541 cgataccgtc gactaaagcc aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa
     3601 gtgatattat ttatttggtt gtaaccaacc aaaagaatgt aaataactaa tacataatta
     3661 tgttagtttt aagttagcaa caaattgatt ttagctatat tagctacttg gttaataaat
     3721 agaatatatt tatttaaaga taattgcgtt tttattgtca gggagtgagt ttgcttaaaa
     3781 actcgtttag atccactagc ttaattaagc atgcactaga taacttcgta tagcatacat
     3841 tatacgaagt tatagcttct gatggaatta gaacttggca aaacaatact gagaatgaag
     3901 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
     3961 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
     4021 atcgaattct agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa
     4081 aaacattttt tttttttact gcactggata tcattgaact tatctgatca gttttaaatt
     4141 tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg
     4201 acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc
     4261 tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa
     4321 tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc
     4381 catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt
     4441 tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat
     4501 cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc
     4561 tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     4621 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     4681 tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt
     4741 ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat
     4801 tttcgactat attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag
     4861 atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt
     4921 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     4981 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     5041 tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     5101 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     5161 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     5221 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     5281 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     5341 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     5401 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     5461 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     5521 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caagccatgg
     5581 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     5641 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     5701 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta accgatccgc
     5761 cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc cacagcgtcg
     5821 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     5881 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     5941 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     6001 attaagggta tctagtatta cataacatct caactcctat ccagcgtggg tgcccagtgt
     6061 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     6121 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta
     6181 gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact ggagcagccg
     6241 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     6301 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     6361 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     6421 tagattttag atagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     6481 tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata ttaatgagat
     6541 gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc
     6601 aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc ttcctgacca
     6661 acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag aatacatttg
     6721 catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag
     6781 ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg
     6841 ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt cacggcgatt
     6901 gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg
     6961 gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc
     7021 tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc
     7081 ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca
     7141 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc
     7201 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag
     7261 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc
     7321 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
     7381 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt
     7441 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
     7501 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
     7561 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
     7621 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
     7681 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
     7741 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
     7801 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa
     7861 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata
     7921 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga
     7981 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc
     8041 tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata
     8101 ataagaatac atttaattta gaaaatgctt ggatttcact ggaactagaa ttcataactt
     8161 cgtatagcat acattatacg aagttatgga tctgagctcc agcttttgtt ccctttagtg
     8221 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10381 atttccccga aaagtgccac
//
