LOCUS       sequence-123636-         834 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of cfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU3
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..82)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(422..455)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        459..506
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ggatatctgc agaattcgcc cttatgagta aaggagaaga acttttcact ggagttgtcc
       61 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      121 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      181 tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
      241 ttcagccaac acttgtcact actttctcat ggggcgttca atgcttctcg agatacccag
      301 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      361 gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta
      421 cgaagttcct atactttcta gagaatagga acttccctgt tgacaattaa tcatcggcat
      481 agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg
      541 agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
      601 accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
      661 ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gttgtgcacc acgcgatgac
      721 cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcgat
      781 gcgcccattg tcgcacatga aaactatcaa tgggctaact acgttcgtgg cgtg
//
