LOCUS       sequence-123642-        1053 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of mChO (mCherry variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU4
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(497..530)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        534..581
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttatggtct caaagggtga agaagataac atggcaatta ttaaagagtt
      121 tatgcgtttc aaggtgcata tggagggatc tgtcaatggg catgagtttg aaattgaagg
      181 tgaaggagaa ggccgaccat atgagggaac acaaaccgca aaactaaagg taagtttaaa
      241 catatatata ctaactaacc ctgattattt aaattttcag gtaactaaag gcggaccatt
      301 accattcgcc tgggacatcc tctctccaca gttcatgtat ggaagtaaag cttatgttaa
      361 acatccggca gatataccag attatttgaa actttcattc ccggagggtt ttaagtggga
      421 acgcgtaatg aattttgaag acggaggagt tgttacagtg acgcaagact caaggtaagt
      481 ttaaacagtt cggtacgaag ttcctatact ttctagagaa taggaacttc cctgttgaca
      541 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc
      601 aggaggcaga tcatgagtct gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc
      661 taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacc
      721 gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagttgt
      781 gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa tcagctcgac
      841 gagttttccc tcgatgcgcc cattgtcgca catgaaaact atcaatgggc taactacgtt
      901 cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggcgt ggacatggtg
      961 atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg
     1021 gtcggaaccg tattgcagca gctttatcat ctg
//
