LOCUS       sequence-123853-        1085 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a fluorescent chitin reporter under Gal4 control in 
            Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Sobala LF, Wang Y, Adler PN
  TITLE     ChtVis-Tomato, a genetic reporter for in vivo visualization of 
            chitin deposition in Drosophila.
  JOURNAL   Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. 
            Epub 2015 Sep 22.
  PUBMED    26395478
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.126987"; journalName: "Development"; date: "2015-11-15-
            15"; volume: "142"; issue: "22"; pages: "3974-81"
COMMENT     Sequence Label: pWalium-ChtVis-Tomato
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..185
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(198..231)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    247..341
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(354..387)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    405..835
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 gtgttcactt tgcttgtttg aattgaattg tcgctccgta gacgaagcgc ctctatttat
       61 actccggcgc tcgctgtcga cgagtctccg ctcggaggac agtactccgc tcggaggaca
      121 gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg gaggacagta
      181 ctccgacctg cggatccata acttcgtata gcatacatta tacgaagtta tccatgggag
      241 tctccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgga ggacagtact
      301 ccgctcggag gacagtactc cgctcggagg acagtactcc gacctgcctg cagataactt
      361 cgtatagcat acattatacg aagttataag ctggtactac tagtgttgtt ggttggcaca
      421 ccacaaatat actgttgccg agcacaattg atcggctaaa tggtatggca agaaaaggta
      481 tgcaatataa taatctttta ttgggtatgc aacgaaaatt tgtttcgtca acgtatgcaa
      541 tattctttat taaaagaggg tatgcaatgt attttattaa aaacgggtat gcaatataat
      601 aatcttttat tgggtatgca acgaaaattt gtttcgtcaa agtatgcaat attttttatt
      661 aaaagagggt atgcaatgta ttttattaaa aacgggtatg caataaaaaa ttatttggtt
      721 tctctaaaaa gtatgcagca cttatttttt gataaggtat gcaacaaaat tttactttgc
      781 cgaaaatatg caatgttttt gcgaataaat tcaacgcaca cttattacgt ggccaactag
      841 tgtaccgaat taggccttct agtggatcaa ttcggcttgt cgacatgccc gccgtgaccg
      901 tcgagaaccc gctgacgctg ccccgcgtat ccgcaccagc cgacgccgtc gcacgtcccg
      961 tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg
     1021 cgttcgccgg gatcaactac cgccacctcg acccgttcat catgatggac cagatgggtg
     1081 aggtg
//
