LOCUS       sequence-123932-         354 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ145
FEATURES             Location/Qualifiers
     source          1..354
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..50)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(32..50)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(60..77)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(60..76)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..91)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(294..314)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
       61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgccggt
      241 ctcgatagcc accacttggg gatcctgcgt tatctagagg gaacgccatg gggcaacttt
      301 tgtatacaaa gttggcatta taaaaaagca ttgctcatca atttgttgca acga
//
