LOCUS       sequence-123975-         960 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  luciferase reporter in lentivirus backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     Dux4 reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRRLSIN.cPPTLuciferase.WPRE
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    89..206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      275..284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(347..365)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
       61 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
      121 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      181 aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag atctgcgatc
      241 taagtaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      301 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      361 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      421 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      481 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      541 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      601 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      661 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
      721 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      781 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
      841 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
      901 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
//
