LOCUS       sequence-124167-         771 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Reporter Plasmid - deGFP driven by a qacR responsive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES001 (pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..224
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             261..283
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(584..603)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
       61 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      121 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      181 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg aacaacgcca
      241 gtgaaaagct ccatggtata tctccttctt aaagttaaac aaaattattg cttttaagtt
      301 ccctccaatc cttatagacc gtgcgatcgg tctataagga ttataatcta tttttttaca
      361 tattacaaca aaaaatcttg cgaaatttaa atgatgctat ggatgtgaga ccttgatcgg
      421 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      481 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      541 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      601 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      661 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      721 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat g
//
