LOCUS       sequence-124379-         776 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of nucleotide free Rac1 in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Garcia-Mata R, Wennerberg K, Arthur WT, Noren NK, Ellerbroek SM, 
            Burridge K
  TITLE     Analysis of activated GAPs and GEFs in cell lysates.
  JOURNAL   Methods Enzymol. 2006;406:425-37.
  PUBMED    16472675
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2006"; volume: "406"; pages: "425-37"
COMMENT     Sequence Label: pGEX-4T1-Rac1 G15A
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..605
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVAKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVSLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(671..693)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tggttccgcg tggatccccg gaattcatgc aggccatcaa gtgtgtggtg gtgggagacg
       61 gagctgtagc taaaacttgc ctactgatca gttacacaac caatgcattt cctggagaat
      121 atatccctac tgtctttgac aattattctg ccaatgttat ggtagatgga aaaccggtga
      181 atctgggctt atgggataca gctggacaag aagattatga cagattacgc cccctatcct
      241 atccgcaaac agatgtgtcc ttaatttgct tttcccttgt gagtcctgca tcatttgaaa
      301 atgtccgtgc aaagtggtat cctgaggtgc ggcaccactg tcccaacact cccatcatcc
      361 tagtgggaac taaacttgat cttagggatg ataaagacac gatcgagaaa ctgaaggaga
      421 agaagctgac tcccatcacc tatccgcagg gtctagccat ggctaaggag attggtgccg
      481 taaaatacct ggagtgctcg gcgctcacac agcgaggcct caagacagtg tttgacgaag
      541 cgatccgagc agtcctctgc ccgcctcccg tgaagaagag gaagagaaaa tgcctgctgt
      601 tgtaactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg
      661 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      721 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcgg
//
