LOCUS       sequence-124392-         860 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Express soluble His-tagged zebrafish thrombopoietin in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Svoboda O, Stachura DL, Machonova O, Pajer P, Brynda J, Zon LI, 
            Traver D, Bartunek P
  TITLE     Dissection of vertebrate hematopoiesis using zebrafish 
            thrombopoietin.
  JOURNAL   Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. 
            Epub 2014 May 28.
  PUBMED    24869937
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-03-564682"; journalName: "Blood"; date: 
            "2014-07-10- 10"; volume: "124"; issue: "2"; pages: "220-8"
COMMENT     Sequence Label: pAc-­His-­zfTpo
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatggctagc caccatcacc atcaccatgg tggatccact agaccaattg actttgtgtg
       61 tgacagcgag gccaggagag tgatgaacaa ggtgaaggat ctacaagaag aaaaggtaat
      121 ctgcagtggc gtagacgctc tcccctcttc tattcaatta ccatgcatca gcattcacaa
      181 agcaacctgg gagagaaaat cagtacagga gcgcagggcc gagatcctgc tgtctttggg
      241 gactctagcg caggatgttc ggtctgccag gacactcagc caacctggct gtggactcac
      301 tctgctgaag cgcttggaac acagtatcaa caactacctg catgtagtca gactgctcca
      361 cgtggagcag ggggagcagg aagggccagc ggtagagagg tgtctgggtc agccctcgaa
      421 ggatttgggt ttggtgctga agtactttgg gcttctgctc acaggcaagc tagagttgct
      481 cattgcagag atggcaaaat ggtgctaggg atcccacagg acgggtgtgg tcgccatgat
      541 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
      601 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
      661 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
      721 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
      781 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
      841 taaactaccg cattaaagct
//
