LOCUS       sequence-124418-         979 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent
            protein-based calcium integrator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, 
            Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER
  TITLE     Neural circuits. Labeling of active neural circuits in vivo with 
            designed calcium integrators.
  JOURNAL   Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922.
  PUBMED    25678659
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-02-13- 13"; volume: "347"; 
            issue: "6223"; pages: "755-60"
COMMENT     Sequence Label: pcDNA3-CaMPARI
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..619
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccac catgctgcag aacgagcttg ctcttaagtt
      121 ggctggactt gatattaaca agactggagg ttctcatcat caccaccacc atggatccga
      181 ccaactgact gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga
      241 cggggatggg acaataacaa ccaaggagct ggggacggtg atgcggtctc tggggcagaa
      301 ccccacagaa gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac
      361 aatcgacttc cctgagttcc tgacaatgat ggcaagaaaa atgaaagaca cagacagtga
      421 agaagaaatt agagaagcgt tccgtgtgtt tgataaggat ggcaatggct acatcagtgc
      481 agcagagctt cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga
      541 tgaaatgatc agggaagcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt
      601 acaaatgatg acagcgaagc tcgagtgcga gaaaatctat gtgcgtgatg gagtgctgac
      661 gggtgatatt catatgatct tgttgcttga aggaaatgcc cattaccgat gtgacttcag
      721 aactacttac aaagctaagg agaagggtgt caagttacca ggcgtgcact ttgtggacca
      781 ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagtatgc
      841 tgttgctcat tctggattgc ctgacaatgc cagacgaggc ggtaccggcg gatccatggt
      901 gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca
      961 ccactttgtg atcgacgga
//
