LOCUS       sequence-124430-         624 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  for transgenic animals.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Kobayashi I, Kobayashi-Sun J, Kim AD, Pouget C, Fujita N, Suda T, 
            Traver D
  TITLE     Jam1a-Jam2a interactions regulate haematopoietic stem cell fate 
            through Notch signalling.
  JOURNAL   Nature. 2014 Aug 21;512(7514):319-23. doi: 10.1038/nature13623. Epub
            2014 Aug 13.
  PUBMED    25119047
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13623"; journalName: "Nature"; date: "2014-08-21- 
            21"; volume: "512"; issue: "7514"; pages: "319-23"
COMMENT     Sequence Label: bactin-loxp-BFP-loxp-dsred-pBSII
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    343..464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(380..399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     434..453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(465..498)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacta cttttaaatc
       61 cgagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
      121 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
      181 atactgcgac ctccctagca aactggggca caagcttaat taggcgcgcc actctagatc
      241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      301 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaataact tcgtatagca
      481 tacattatac gaagttatcg atccatgtcg actagtccac catggcctcc tccgaggacg
      541 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
      601 tcgagatcga gggcgagggc gagg
//
