LOCUS       sequence-124661-        1148 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Transient expression of a minimal sgRNA targeting the GLuc reporter,
            in mammalian cells. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_sgRNA
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(44..119)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(199..218)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(382..399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(382..398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(607..626)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     726..748
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(786..804)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        872..976
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcaggttta aacagtcgac tctagactta attaagaaaa aaagcaccga ctcggtgcca
       61 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
      121 tagtgagtcg tatctagatc ggtgtttcgt cctttccaca agatatataa agccaagaaa
      181 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
      241 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
      301 gtgtttacag tcaaattaat tccaattatc tctctaacag ccttgtatcg tatatgcaaa
      361 tatgaaggaa tcatgggaaa cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      541 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      601 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      661 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      721 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      781 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      841 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      901 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1141 tgagagtt
//
