LOCUS       sequence-124725-       11813 bp    DNA     circular SYN 15-APR-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox-controllable 
            expression of FLAG-HA-tagged murine Dnd1 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-Dnd1-IN
FEATURES             Location/Qualifiers
     source          1..11813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3488..3497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3497..3520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3521..3547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4671..5245,5246..5248,5249..5257)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4838..4855)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5065..5084
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5258..6052
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5312..5331)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5922..5941
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6375..6408)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6428..6608
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6633..6651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6634..6651)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6941..6960)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7174..7193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7293..7315
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7353..7371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7439..7543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7544..7612,7613..8404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7762..7781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8575..9163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9064..9083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9317..9334
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9404..9424)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9409..9738
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9589..9724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10872..10937
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11067..11087
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11512..11646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11549..11568)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11603..11622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgagggcc accatggact acaaggacga cgatgacaag tacccttatg acgtgcccga
     3541 ttacgcttct ggtggcggtg gctcgggcgg aggtgggtcg ggtggcggcg gatcaggatc
     3601 catgcagtcc aaacgggagt gcgagcaatg gtgtgagagg gtgaatccag agaacaaggc
     3661 agccctggag gcgtgggtaa gggagactgg catccgcctg gtgcaggtca acgggcaaag
     3721 gaagtatgga gggccacccc caggctgggt gggcagcccg ccaccgtcag ggtccgaggt
     3781 gtatatcgga cgacttccgc aggacgtgta cgagcaccag ctaatcccac tcttccagcg
     3841 cgtggggcgc ctctatgagt tccgcctaat gatgaccttc agtggtttga accgcggctt
     3901 tgcctatgcg cgctacagct cgcggcgcgg tgcccaggcc gccattgcta cgctgcacaa
     3961 ccaccagctg cggccttctt gccagctcct ggtgtgccga agcaccgaga agtgcgagct
     4021 gacggtggac gggctgcctc ttagcttaaa ccgccgagcg ttactgctcg ccctgcaacc
     4081 gttcggtccc tgcctccagg agacactgct gctacccagc cctgggtctg cgccctctca
     4141 gattgcgctg ctcaagttca gtacgcaccg agctgctgct atggccaaaa aggccctggt
     4201 agaaggtcag tcacgcctct gtggagagca ggtggctgtg gagtggctca agccagacct
     4261 aaagcagcac ttccgccagc agcttgcagg tccctcactg cggttcctca ggccagatgt
     4321 cagccaacta acccagacca gggagaagct ggggtcccaa ggggctcggg ctgccctgca
     4381 actgctgtgt caacggatga agctaggcag cccagtgttc ctgaccaagt gtttaggaac
     4441 agggcctgct ggctggcatc gcttttggta ccaggtggtg atccctgggc atccagtgcc
     4501 tttcagtggc ctcatttggg ttgtgttggc ctctgattgg caggatgggc atgaggtggc
     4561 caaggatgct gtgtccgcac agctgctgga ggcactgagt gaacctagga ccagcttgtg
     4621 gtctcctggg gctgaagcag gtactatggt taagcagtga ggatcctaac cccctctccc
     4681 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4741 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     4801 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     4861 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     4921 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     4981 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5041 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5101 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5161 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5221 ttcctttgaa aaacacgatg ataatatggc cacaaccatg attgaacaag atggattgca
     5281 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5341 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5401 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5461 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5521 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5581 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5641 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5701 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5761 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5821 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5881 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5941 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6001 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagaattcat
     6061 attaccccta acacctgcca ccccagtctt aatcagtggt ggaagaacgg tctcagaact
     6121 gtttgtctca attggccatt taagtttaat agtaaaagac tggttaatga taacaatgca
     6181 tcgtaaaacc ttcagaagga aagaatgttg tggaccattt tttttgtgtg tggcagtttt
     6241 aagttattgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6301 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc
     6361 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc
     6421 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6481 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6541 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6601 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     6661 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     6721 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     6781 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     6841 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     6901 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     6961 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     7021 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     7081 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     7141 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8701 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8941 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     9001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9361 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     9421 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9481 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9541 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9661 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9721 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     9781 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     9841 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     9901 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     9961 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
    10021 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
    10081 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
    10141 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
    10201 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
    10261 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
    10321 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
    10381 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
    10441 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
    10501 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
    10561 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
    10621 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
    10681 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
    10741 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
    10801 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
    10861 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
    10921 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
    10981 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
    11041 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
    11101 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
    11161 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
    11221 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
    11281 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
    11341 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
    11401 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    11461 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    11521 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11581 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11641 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11701 attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt
    11761 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tag
//
