LOCUS       sequence-124794-        8776 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of human HOXB13 from CMV promoter, puromycin selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas 
            P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, 
            Kantoff PW, Brown M, Loda M, Long HW, Freedman ML
  TITLE     The androgen receptor cistrome is extensively reprogrammed in human 
            prostate tumorigenesis.
  JOURNAL   Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 
            Oct 12.
  PUBMED    26457646
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3419"; 
            journalName: "Nat Genet"; date: "2015-11"; volume: "47"; issue: 
            "11"; pages: "1346-51"
COMMENT     Sequence Label: pLX302_HOXB13
FEATURES             Location/Qualifiers
     source          1..8776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    597..621
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1480..1504)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1506..1547
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1589..2177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1642..1662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2060..2071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2249..2482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2560..2681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2597..2616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2651..2670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2721..2856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2876..2895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2877..2895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2905..2922)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2905..2921)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2914..2936)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3063..3518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3150..3169)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3360..3381
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3544..3648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3649..3717,3718..4509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3867..3886)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4680..5268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5169..5188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5422..5439
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5556..5577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5592..5597,5598..5615,5616..5622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5630..5646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5635..5657
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5654..5670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5654..5670
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5689..5709
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5691..5709
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5737..5963
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5964..6144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6191..6316
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6809..7042
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7227..7271
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7446..7946
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7865..7883
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7968..8567
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7968..7987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8464..8484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8609..8726
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg ggatcaacaa
      601 gtttgtacaa aaaagcaggc ttcatggagc ccggcaatta tgccaccttg gatggagcca
      661 aggatatcga aggcttgctg ggagcgggag gggggcggaa tctggtcgcc cactcccctc
      721 tgaccagcca cccagcggcg cctacgctga tgcctgctgt caactatgcc cccttggatc
      781 tgccaggctc ggcggagccg ccaaagcaat gccacccatg ccctggggtg ccccagggga
      841 cgtccccagc tcccgtgcct tatggttact ttggaggcgg gtactactcc tgccgagtgt
      901 cccggagctc gctgaaaccc tgtgcccagg cagccaccct ggccgcgtac cccgcggaga
      961 ctcccacggc cggggaagag taccccagcc gccccactga gtttgccttc tatccgggat
     1021 atccgggaac ctaccagcct atggccagtt acctggacgt gtctgtggtg cagactctgg
     1081 gtgctcctgg agaaccgcga catgactccc tgttgcctgt ggacagttac cagtcttggg
     1141 ctctcgctgg tggctggaac agccagatgt gttgccaggg agaacagaac ccaccaggtc
     1201 ccttttggaa ggcagcattt gcagactcca gcgggcagca ccctcctgac gcctgcgcct
     1261 ttcgtcgcgg ccgcaagaaa cgcattccgt acagcaaggg gcagttgcgg gagctggagc
     1321 gggagtatgc ggctaacaag ttcatcacca aggacaagag gcgcaagatc tcggcagcca
     1381 ccagcctctc ggagcgccag attaccatct ggtttcagaa ccgccgggtc aaagagaaga
     1441 aggttctcgc caaggtgaag aacagcgcta ccccttaaga cccagctttc ttgtacaaag
     1501 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     1561 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     1621 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1681 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1741 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     1801 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     1861 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     1921 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     1981 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2041 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2101 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2161 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2221 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2281 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2341 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2401 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2461 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     2521 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     2581 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2641 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     2701 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2761 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2821 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     2881 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2941 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3001 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3061 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3121 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3181 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3241 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3301 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3361 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3421 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3481 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3541 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3601 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3661 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3721 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3781 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3841 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3901 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3961 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4021 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4081 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4141 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4201 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4261 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4321 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4381 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4441 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4501 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4561 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4621 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4681 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4741 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4801 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4861 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4921 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4981 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5041 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5101 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5161 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5221 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5281 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5341 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5401 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5461 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5521 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5581 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5641 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     5701 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     5761 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     5821 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     5881 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     5941 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6001 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6061 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6121 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6181 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6241 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6301 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6361 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6421 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6481 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6541 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     6601 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     6661 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6721 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6781 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     6841 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     6901 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     6961 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7021 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7081 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7141 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7201 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7261 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7321 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7381 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7441 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     7501 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     7561 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     7621 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     7681 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     7741 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     7801 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     7861 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     7921 accgaatcac cgacctctct ccccaggggg taccaccgga gcttaccatg accgagtaca
     7981 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     8041 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     8101 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8161 gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg
     8221 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     8281 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8341 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8401 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8461 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8521 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagaa ttctagatct
     8581 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     8641 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     8701 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     8761 ggcgccggct cgaggg
//
