LOCUS       sequence-124986-        1279 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pSTC2,cisRaj12-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKbreak12
FEATURES             Location/Qualifiers
     source          1..1279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        139..173
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     protein_bind    428..444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             491..502
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             518..1228
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 gaattctcta gaaaaaaaaa tccttagctt tcgctaagga tagctcgtca ctgtccaggt
       61 tcagggtttc ccgtgctcag tatctctatc actgataggg atgtcaatct ctatcactga
      121 tagggatcta gaatcgattt gacagctagc tcagtcctag gtataatgct agcggcgctg
      181 ccttcgggcg accctgatga gcttgagttt agctcgtcac tgtccaggtt caatcaggcg
      241 aaacggtgaa agccgtaggt tgcccgacgg gcaggaagaa gggttccttt gagcgaatct
      301 agcggcacct cgctaggatt tgctcgaagg gattctgggc aaagcccgcc gaaaggcggg
      361 cttttctgta ctagtctgca gggatcgacg agagcatgca tataaatgtg agcggataac
      421 attgacattg tgagcggata acaagatact gagcacaccc agtatcattc tcttcttcct
      481 gcccacgcgg aaagaggaga aaggtgtaat gactagtcgt aaaggcgaag agctgttcac
      541 tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt
      601 gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac
      661 tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca
      721 gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc
      781 ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg
      841 tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga
      901 ctttaaagaa gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa
      961 tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca
     1021 caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg
     1081 tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa
     1141 agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat
     1201 cacgcatggt atggatgaac tgtacaaagc tagcgcagcg aacgacgaaa attacgccct
     1261 tgcagcgtaa taactgcag
//
