LOCUS       sequence-125085-         590 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  8x Target 2 0gap with SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-321
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(197..216)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    262..317
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(316..335)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgcctcatca
       61 ttttggcaaa gaattgatta attcgagcga acgcgtagcc aaaggcaaca ggcggccgca
      121 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
      181 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
      241 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
      301 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
      361 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
      421 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
      481 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
      541 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
//
