LOCUS       sequence-125138-         857 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Second generation Rac1 FRET biosensor for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002."
COMMENT     Sequence Label: pLenti-Rac1-2G
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..626)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(645..666)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac gaattctggt gctcgagtca caacagcagg
       61 cattttctct tcctcttctt cacgggaggc gggcagagga ctgctcggat cgcttcgtca
      121 aacactgtct tgaggcctcg ctgtgtgagc gccgagcact ccaggtattt tacagcacca
      181 atctccttgg ccatagctag accctgcgga taggtgatgg gagtcagctt cttctccttc
      241 agtttctcga tcgtgtcttt atcatcccta agatcaagtt tagttcccac taggatgatg
      301 ggagtgttgg gacagtggtg ccgcacctca ggataccact ttgcacggac attttcaaat
      361 gatgcaggac tcacaaggga aaagcaaatt aagaacacat ctgtttgcgg ataggatagg
      421 gggcgtaatc tgtcataatc ttcttgtcca gctgtatccc ataagcccag attcaccggt
      481 tttccatcta ccataacatt ggcagaataa ttgtcaaaga cagtagggat atattctcca
      541 ggaaatgcat tggttgtgta actgatcagt aggcaagttt tacctacagc tccgtctccc
      601 accaccacac acttgatggc ctgcatggta cccccggcgg cggtcacgaa ctccagcagg
      661 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
      721 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      781 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
      841 ttcttctgct tgtcggc
//
