LOCUS       sequence-125329-         779 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pBAD etuAld1-20- gfpmut2-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ129
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..762)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(52..762)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(92..112)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     707..735
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccaagcttt taagctgcta aagcgtagtt ttcgtcgttt gctgcgtcga ctttgtatag
       61 ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtgatc
      121 tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg
      181 taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac
      241 gcttccatct tcaatgttgt gtctaatttt gaagttaact ttgattccat tcttttgttt
      301 gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat
      361 gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc
      421 ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct
      481 ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgctgtt tcatatgatc
      541 tgggtatctc gcaaagcatt gaagaccata cgcgaaagta gtgacaagtg ttggccatgg
      601 aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc
      661 accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac
      721 aagaattggg acaactccag tgaaaagttc ttctccttta cttctagatt tctttgctt
//
