LOCUS       sequence-125416-        7094 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pP2A11  (GB0082)
FEATURES             Location/Qualifiers
     source          1..7094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4144..4162)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4145..4162)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4180..4196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4180..4196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4193..4215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4204..4220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4228..4234,4235..4252,4253..4258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4273..4294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4411..4428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4582..5170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4662..4681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5341..6132,6133..6201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5964..5983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6202..6306)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6384..6839)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6521..6542)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6733..6752
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6965..6987
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6979..6996
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6980..6996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7003..7022
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7003..7021
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7052..7061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7056..7065
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggaggt caaacaattt tgaggaattc aatttcatct ttagctggta caataatcat
       61 tttatcatga aaataagcag ctcaagagaa tttttgaaga atcttttatt tctttaacat
      121 ttaaccacat gaatttttaa tttttttttg caatacattt aaaccgaaat ggtcaaacga
      181 tcaaccaact gatctttatt ctaataaact tctagtttac atttgcatgt gagtgcatca
      241 tcattatcat atttgtacac aacaaacaag aaaaagatat aaacaatatt ttatttaaat
      301 atttatattc cactttgact gtagatatta aatcttgtca tcatttatag tctcaatatt
      361 ataatttttt tattttttca aaattcaaaa gtttacaatt attttttgaa ctataatatt
      421 atccaagatg aacatctcaa gaagaaaatt attaatattg ttatggttaa aattttacat
      481 acaatacttg ttttttgctt tacttttatc ttaccgtaga tacacaatcg acgataactt
      541 agtgatcaca caataataat tattttgttc atgacacaat atttataaga aatacttatt
      601 tctttctttt atccttcagt agttcataat aaaaacatac cataatattt gtgatgcatt
      661 catagtacgt aatgaaatga caatttatgt caaattattt tcttttatac tctcaaacct
      721 ctcgtaaagg tgagatgagt catttatcca attatacata aatatgtctt tattcatgct
      781 ctttatcaca ttctgacaca ttcacttaat ttcaagagta agcaagcatg ataactgaaa
      841 ctatttatgc gtatcttacc ttgatatttg acacattaca tgacacacct caacatcact
      901 ttcaaagatt aagcgcacca ccatattatc tttctttttt tttttatgaa ggttttataa
      961 aattattaaa ttaggtccaa aaaattgttt gtcaaataac cttttatact agattgatga
     1021 caaaaattac ctttacgttt tgaaagacca ttttaagacc taatctatca gtgactcctt
     1081 aaagttggca caatatttca cttagacacc ctaattgaat gatgttcatt ttaaacaccc
     1141 aatgtagggt tccgctatat cattttgaca catttcttaa catcaacaaa aatatataat
     1201 gagtatgtga tatactcgcg aatgacgtga aaaatgaaga catttgttat ttgtatcaaa
     1261 gtagttacta aataattaat tttgaataaa aataaaagct gaccagtaaa tcaataacac
     1321 ataatatttt ccacctaata attaaaatat aaaataaaaa agagccatct cagggtcatc
     1381 tgcccaccat tgctatttca aagaaatttg tacgttagtt tatagaaatt gatgttaaaa
     1441 ttctttcaag aaaaatttat gaatgaattt attctctaat ttaaaaatat tttctgttat
     1501 ttttgttgaa agaaatttaa cttggataaa atggtggtta aaactggaaa gaagaaaaga
     1561 gaaaaaataa ttaaaaatca tttcacgctc taatcaatga gcgtatcaca ttcattatgt
     1621 tatataagca aaagtgacaa aacgaaaata atatattaca tgaaatgtct aaaataaata
     1681 tcgtctaatt aaaatatcta agtaacatat tgtgcctaac tttagaggga tcatcaataa
     1741 gttaaacccc attttaataa ctcataattg tcctttttat ttaatattgt cacaaatcac
     1801 aatgataatt aacattaatt tgtcctttgt gacgtccata ttcatgcatt taaccaatca
     1861 tcttcatttg gacttattat cacaattatc ccactttcct cacaaaatgg agcattcaag
     1921 tggaatagac tacacgattt ttaatttcat caaaaacatc tttttgcttt attcattatt
     1981 atattgtcgc tattgttgaa ttttatttgc cctaaatttc ttaccataaa tagatttttc
     2041 ttttagaaaa aggagattga ctaattcttt tcttgtagga aaaggtttag gactctataa
     2101 atagagacat attccttcta acttaatcaa catttacaat gtagtcttaa agactttgaa
     2161 agtttttggt tagggggaga aattgtgggt cacaagcttg atacgttatc aattgtgtaa
     2221 acctcccatg tattctgagt gaatttggtt gaggttgttt ccctctgtat tttgtactct
     2281 catatttata gtggattgtt catctctttc gtggacgtag gtcgattgac cgtcgattga
     2341 ccgaaccacg ttaaatcttt gtattttttg atatatttct cattatcttc ttactcgtga
     2401 tctttcaagg tttgcattgc tatcttccgc gttacaccaa cttatttacg atcctaacag
     2461 ctatggtgtg gaaacataaa tcaaacattt tactgatata aacacatctt tgattataac
     2521 atgatagaaa tttgagccca actttttatc atcattatat acaaaaagtt ctaaattttt
     2581 tttttgatgt agtaaaactt aaatccatag tcttgcccct aaaccaatga cataatatat
     2641 aacccaaaat atactagttt tcgccctcga gccctttaaa aagtatagtc aatatttacg
     2701 gtgaccgtga atttcttaat tatgatatat aatttaaaag aaatcatgat cacattctac
     2761 tgatgagaac atgtgctaat caagggaaaa catggatgtg aaaaatactt tttgttaaaa
     2821 gtaaaaaaaa atgtgaaatt ttgttagtta tttactacct atacattatt tgagcatgtg
     2881 caaactttac aaatacctaa tagaagattt tcacctgcct gtatatatgt aaattaatta
     2941 taatgaacac tctcacataa aataattatc agtatataca ttaatacttg ccctccacaa
     3001 tgaattaaat aaaatgtaga acatgatcta cacttcaata aaactaagac cataaagaat
     3061 aatttcaaaa tatacacatg tcaacaataa attatttgca tattatatta acttactaaa
     3121 caatctttac ttttgaaata taaaaataat caagttataa gtctgctcaa agtaaagcac
     3181 ttgttagact catctgattt tgagaaggta agcaaattga tggtgcataa tagtcacaag
     3241 taaaatataa aatagatttc attagtaaaa ttgtttttta ctttctttat atataattat
     3301 caatatcctt caatggtagg ttaattatat tgttaacttc ttgttgaatt aaagcaataa
     3361 gacaagaata ttaaagataa aagaacaata aaaatagaaa gactaagaga taagagtttt
     3421 cttattcttc tttcaataag tatcatcaag tgtatacaat ataaattttt gtatttttga
     3481 tctatctatt tataatgtta tatataagca tacaaaagat cagtcataaa tatgacttta
     3541 atcatgaaaa taatgaaaga gattatgaag gcgtaaggtt actagaataa tagtcattaa
     3601 aaaaaggggt tatctttata attgaataat tgatgaagta atggagataa ttagtgagca
     3661 taaatttttt taaaaaaatg gacatttaca ctataatatt ttataacact ttcccttaaa
     3721 catctaggta taaataatga gtcttgtcaa aatcttagta ggaaaaattc tgtgaaattt
     3781 ttttagtgaa aacaaatgat ataaatatct tgaatactca ttatttgttg tctcattaaa
     3841 aatcttatct gacctataaa ataaattatt tgctcaactc aaaatagttt ttcattctaa
     3901 aattagtata attattagtg aatatttaat taacataatt gtatactaag gggcctataa
     3961 attggattct tctcaaagaa aaataaaatc accacacaac tttcttcttc tgctcatcaa
     4021 ttagcaatta atccaaaacc aaatgcgaga ccactcatcg cccaatcact agtgaattcg
     4081 cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag
     4141 tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6301 tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg
     6361 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     6421 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6481 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6541 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6601 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6661 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6721 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6781 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     6841 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6901 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6961 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     7021 ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt
     7081 gggcgatgag tggt
//
