LOCUS       sequence-125441-        1118 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses a neomycin tagged codon optimized L1PA4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM
  TITLE     Molecular reconstruction of extinct LINE-1 elements and their 
            interaction with nonautonomous elements.
  JOURNAL   Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. 
            Epub 2012 Aug 23.
  PUBMED    22918960
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/molbev/mss202"; journalName: "Mol Biol Evol"; date: 
            "2013-01"; volume: "30"; issue: "1"; pages: "88-99"
COMMENT     Sequence Label: pBS-L1PA4CHmneo
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     286..305
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
       61 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      121 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
      181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
      241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
      301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
      361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
      421 atcggcaata aaaagacagg aataaaacgc acggccggcc agctaggaat tcttagttgc
      481 cgccgatcag gctgaacatc cggtgcttgg tcttctggcc ctggctcagc ttgctcagga
      541 tgatggtctc cagcttcatc caggtgccca cgaagctcat gaactcgtcg ttcttgatgg
      601 cggcgtagta ctccatggtg tagatgtgcc acatcttctt gatccagtcg atcatggtgg
      661 ggcacttggg ctggttccag gtcttggcga tggtgaacag ggcggcgatg aacatccggg
      721 tgcaggtgtc cttgtagcag cagctcttgt agtccttggg gtagatgccc agcaggggga
      781 tggcggggtc gaaggggatc tccagctcca ggtcccgcag gaaccgccac acgctcttcc
      841 acaggggctg caccagcttg cagtcccacc agcagtgcag cagggtgccg atctcgccgc
      901 agccccgcca gcaccggttg ttgccgctct tcttgatgat ggccatccgc acgggggtca
      961 ggtggtaccg catggtggtc ttgatctgca tctcccggat ggccaggctg ctgctgcact
     1021 tcttcatgtg cttcttggcg gcgtagatgt cctccttgct gaagtgccgg ttcatgtcct
     1081 tggcccactt cttgatgggg ttgttggtct tcttcttg
//
