LOCUS       sequence-125573-         921 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses humanized BpCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY006 (pcDNA3.1-hBpCpf1)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttcacggc gatatcctgg gcctgcatgt aattcaggaa ggcgtccaga aaatcgccgt
      241 cgcctctata ctcctgcttg aacatgaact tcttgccgaa gttgtatgtg gtgttcttgc
      301 tcacgtcgat ctctgtgccg gtgatggggc tccggtagat cttctcgccg tccttctttg
      361 tgatgatgcc atacagggcg ttggcgaacc acttgtagat cagctccacg gacttgccgc
      421 tgatggcggt cttatccaga aacttctcgg ccatattgcc atcgaacacg gccttgtcct
      481 ccagcagggc tgggatgatc tgctctctca cgatgtcgtt cacgtacacg tactcgccct
      541 tatccttgtc gaaataggag ccatcgccgg cgatcttcac ggtccactcc ttgcggtcca
      601 gcagcctctt tgtagggtac tcctccaggt taaaggtgaa cagcatctcg ccatcgtcga
      661 ttctgatgtc cttcatcttg gcaaagaact gtctcttaga ctgcattgtg atcagccggg
      721 tcttatcgaa cacgtccaca aagccggtat cagggcacag ggtccgggta taggcgttat
      781 tcaggaagta gatcacgccg tcctgataag aattgccgtt attgttggac acctggatgg
      841 ggttagagat ggagccggcc tcgccctcct tgattgtatt aaaagacagg tcggacattc
      901 tggcgcacag ccgggcctca a
//
