LOCUS       sequence-125664-         917 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses humanized PcCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY017 (pcDNA3.1-hPcCpf1)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttgtcctt ctcgtaggat ctctcctgca caaactgcag ccactccttg ttagagatgg
      241 ccagcttcag cttgccgccc tcggaggtct gtctgatctg cctcagtgcc cacaggccct
      301 tcagggcgat attataggct ccgtttgcat ctgcgtcctt gggcaggctc ttattgccct
      361 cgcgtgtgtc aaagaacctg ccatcggcgc cggccactgg agagatcagg taatccaggt
      421 ccttctcctt ccaggagttt ctcatctgca cggtcagctt gaacagcttc agcaggtcca
      481 caaagaaatc cttctgcttc tcgtccacga tggcggtttt caggtcggcg gtataatcga
      541 tctcgtaccg cacaaacagg gacttgaagg cctcggtcag ggcaaactcc tcgctatccc
      601 actggccatt cttctgagaa tttctgaagt tcttgatccg ggagccgtgg gtgcacagaa
      661 tccacatgct cctagagccc tcggccttct tggtgaagtt cttatagtca aaggcgaact
      721 caaaccaatc cttcttaggg ttgtaggaga tgctgtcgaa cttctgaaag aagctcttgg
      781 ccttatccac attctcgtac tgcacgtgaa acaggttcac gaagcctgtg gttgggtcga
      841 tattggatgt gttccaggca gggatataga acagaaagcc gttctgcttg cccatctcct
      901 taaagctctt gaaaggg
//
