LOCUS       sequence-125865-        6863 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway ORF clone of human MET [NM_000245.2] without stop codon (for
            C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6863)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E132 Hs.MET-nostop
FEATURES             Location/Qualifiers
     source          1..6863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4354..4453)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4830..5621
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5714..6302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6203..6222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6456..6473
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6632..6659)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6751..6837)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa ggcccccgct gtgcttgcac ctggcatcct cgtgctcctg tttaccttgg
      241 tgcagaggag caatggggag tgtaaagagg cactagcaaa gtccgagatg aatgtgaata
      301 tgaagtatca gcttcccaac ttcaccgcgg aaacacccat ccagaatgtc attctacatg
      361 agcatcacat tttccttggt gccactaact acatttatgt tttaaatgag gaagaccttc
      421 agaaggttgc tgagtacaag actgggcctg tgctggaaca cccagattgt ttcccatgtc
      481 aggactgcag cagcaaagcc aatttatcag gaggtgtttg gaaagataac atcaacatgg
      541 ctctagttgt cgacacctac tatgatgatc aactcattag ctgtggcagc gtcaacagag
      601 ggacctgcca gcgacatgtc tttccccaca atcatactgc tgacatacag tcggaggttc
      661 actgcatatt ctccccacag atagaagagc ccagccagtg tcctgactgt gtggtgagcg
      721 ccctgggagc caaagtcctt tcatctgtaa aggaccggtt catcaacttc tttgtaggca
      781 ataccataaa ttcttcttat ttcccagatc atccattgca ttcgatatca gtgagaaggc
      841 taaaggaaac gaaagatggt tttatgtttt tgacggacca gtcctacatt gatgttttac
      901 ctgagttcag agattcttac cccattaagt atgtccatgc ctttgaaagc aacaatttta
      961 tttacttctt gacggtccaa agggaaactc tagatgctca gacttttcac acaagaataa
     1021 tcaggttctg ttccataaac tctggattgc attcctacat ggaaatgcct ctggagtgta
     1081 ttctcacaga aaagagaaaa aagagatcca caaagaagga agtgtttaat atacttcagg
     1141 ctgcgtatgt cagcaagcct ggggcccagc ttgctagaca aataggagcc agcctgaatg
     1201 atgacattct tttcggggtg ttcgcacaaa gcaagccaga ttctgccgaa ccaatggatc
     1261 gatctgccat gtgtgcattc cctatcaaat atgtcaacga cttcttcaac aagatcgtca
     1321 acaaaaacaa tgtgagatgt ctccagcatt tttacggacc caatcatgag cactgcttta
     1381 ataggacact tctgagaaat tcatcaggct gtgaagcgcg ccgtgatgaa tatcgaacag
     1441 agtttaccac agctttgcag cgcgttgact tattcatggg tcaattcagc gaagtcctct
     1501 taacatctat atccaccttc attaaaggag acctcaccat agctaatctt gggacatcag
     1561 agggtcgctt catgcaggtt gtggtttctc gatcaggacc atcaacccct catgtgaatt
     1621 ttctcctgga ctcccatcca gtgtctccag aagtgattgt ggagcataca ttaaaccaaa
     1681 atggctacac actggttatc actgggaaga agatcacgaa gatcccattg aatggcttgg
     1741 gctgcagaca tttccagtcc tgcagtcaat gcctctctgc cccacccttt gttcagtgtg
     1801 gctggtgcca cgacaaatgt gtgcgatcgg aggaatgcct gagcgggaca tggactcaac
     1861 agatctgtct gcctgcaatc tacaaggttt tcccaaatag tgcacccctt gaaggaggga
     1921 caaggctgac catatgtggc tgggactttg gatttcggag gaataataaa tttgatttaa
     1981 agaaaactag agttctcctt ggaaatgaga gctgcacctt gactttaagt gagagcacga
     2041 tgaatacatt gaaatgcaca gttggtcctg ccatgaataa gcatttcaat atgtccataa
     2101 ttatttcaaa tggccacggg acaacacaat acagtacatt ctcctatgtg gatcctgtaa
     2161 taacaagtat ttcgccgaaa tacggtccta tggctggtgg cactttactt actttaactg
     2221 gaaattacct aaacagtggg aattctagac acatttcaat tggtggaaaa acatgtactt
     2281 taaaaagtgt gtcaaacagt attcttgaat gttatacccc agcccaaacc atttcaactg
     2341 agtttgctgt taaattgaaa attgacttag ccaaccgaga gacaagcatc ttcagttacc
     2401 gtgaagatcc cattgtctat gaaattcatc caaccaaatc ttttattagt ggtgggagca
     2461 caataacagg tgttgggaaa aacctgaatt cagttagtgt cccgagaatg gtcataaatg
     2521 tgcatgaagc aggaaggaac tttacagtgg catgtcaaca tcgctctaat tcagagataa
     2581 tctgttgtac cactccttcc ctgcaacagc tgaatctgca actccccctg aaaaccaaag
     2641 cctttttcat gttagatggg atcctttcca aatactttga tctcatttat gtacataatc
     2701 ctgtgtttaa gccttttgaa aagccagtga tgatctcaat gggcaatgaa aatgtactgg
     2761 aaattaaggg aaatgatatt gaccctgaag cagttaaagg tgaagtgtta aaagttggaa
     2821 ataagagctg tgagaatata cacttacatt ctgaagccgt tttatgcacg gtccccaatg
     2881 acctgctgaa attgaacagc gagctaaata tagagtggaa gcaagcaatt tcttcaaccg
     2941 tccttggaaa agtaatagtt caaccagatc agaatttcac aggattgatt gctggtgttg
     3001 tctcaatatc aacagcactg ttattactac ttgggttttt cctgtggctg aaaaagagaa
     3061 agcaaattaa agatctgggc agtgaattag ttcgctacga tgcaagagta cacactcctc
     3121 atttggatag gcttgtaagt gcccgaagtg taagcccaac tacagaaatg gtttcaaatg
     3181 aatctgtaga ctaccgagct acttttccag aagatcagtt tcctaattca tctcagaacg
     3241 gttcatgccg acaagtgcag tatcctctga cagacatgtc ccccatccta actagtgggg
     3301 actctgatat atccagtcca ttactgcaaa atactgtcca cattgacctc agtgctctaa
     3361 atccagagct ggtccaggca gtgcagcatg tagtgattgg gcccagtagc ctgattgtgc
     3421 atttcaatga agtcatagga agagggcatt ttggttgtgt atatcatggg actttgttgg
     3481 acaatgatgg caagaaaatt cactgtgctg tgaaatcctt gaacagaatc actgacatag
     3541 gagaagtttc ccaatttctg accgagggaa tcatcatgaa agattttagt catcccaatg
     3601 tcctctcgct cctgggaatc tgcctgcgaa gtgaagggtc tccgctggtg gtcctaccat
     3661 acatgaaaca tggagatctt cgaaatttca ttcgaaatga gactcataat ccaactgtaa
     3721 aagatcttat tggctttggt cttcaagtag ccaaaggcat gaaatatctt gcaagcaaaa
     3781 agtttgtcca cagagacttg gctgcaagaa actgtatgct ggatgaaaaa ttcacagtca
     3841 aggttgctga ttttggtctt gccagagaca tgtatgataa agaatactat agtgtacaca
     3901 acaaaacagg tgcaaagctg ccagtgaagt ggatggcttt ggaaagtctg caaactcaaa
     3961 agtttaccac caagtcagat gtgtggtcct ttggcgtgct cctctgggag ctgatgacaa
     4021 gaggagcccc accttatcct gacgtaaaca cctttgatat aactgtttac ttgttgcaag
     4081 ggagaagact cctacaaccc gaatactgcc cagacccctt atatgaagta atgctaaaat
     4141 gctggcaccc taaagccgaa atgcgcccat ccttttctga actggtgtcc cggatatcag
     4201 cgatcttctc tactttcatt ggggagcact atgtccatgt gaacgctact tatgtgaacg
     4261 taaaatgtgt cgctccgtat ccttctctgt tgtcatcaga agataacgct gatgatgagg
     4321 tggacacacg accagcctcc ttctgggaga catcatcaac tttcttgtac aaagttggca
     4381 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     4441 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     4501 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     4561 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     4621 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     4681 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     4741 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     4801 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     4861 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4921 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4981 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     5041 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     5101 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     5161 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     5221 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     5281 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     5341 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     5401 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     5461 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     5521 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     5581 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     5641 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     5701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5821 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     6121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6421 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6481 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6541 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     6601 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     6661 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     6721 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     6781 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     6841 cctggcagtt ccctactctc gcg
//
