LOCUS       sequence-12605-e         918 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Wang Y, Zayas RM, Guo T, Newmark PA
  TITLE     nanos function is essential for development and regeneration of 
            planarian germ cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Apr 3. 104(14):5901-6.
  PUBMED    17376870
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Apr 3. 104(14):5901-6."
COMMENT     Sequence Label: nanos-PCRII
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..33
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     25..42
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     26..42
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagggttttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
       61 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
      121 ctgcagaatt cggcttcttc ctttaagcta taccaagagt ggattgtgac atgcttcttg
      181 atttaatagc aatttgattt cttgtaattt cttccaaaga attatctgac acttgttttg
      241 ttgtattcat agaaatagga caatatttga ttgtatgagc aaagtcacct gtactgttac
      301 acaatgggca tgtataatta cgtagaactg gacatgtaac cttaccattt aggtctttaa
      361 ctttatgact aacatacatt tcaaaaggtt cattgttatt cctacagaag acacatagtt
      421 caatatgact ctctttgcga atttgattag atgttctaag tttgtgcaat aaccttagcc
      481 atatacttct atccagagta atatgctttt caggccatgc ttccaacaat gcaacccatc
      541 gatccaactc ttttgataat tttaaatcgc tgcgaaataa actgtcatta tcttcttcat
      601 ctaacagtga aaaatctgtc tttgcaatat ttttttgcaa taaattcggt aatatacagg
      661 caacatttgt ccttggataa ttcacattgg atttattgct actataagac acattattag
      721 atgtcactga ttgccaaata aatggagacg tattagccat cttcaatgat tcaggataat
      781 ttaacattaa attaagcttt agtgtgtgat gaattaaaac gaaatatgat tcgcctgtgc
      841 gcatgagaag ccgaattcca gcacactggc ggccgttact agtggatccg agctcggtac
      901 caagcttgat gcatagct
//
