LOCUS       sequence-126056-        3950 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway ORF clone of human SPRED2 [NM_181784.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3950)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E323 Hs.SPRED2
FEATURES             Location/Qualifiers
     source          1..3950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1441..1540)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1917..2708
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2801..3389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3290..3309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3543..3560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3719..3746)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3838..3924)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgac cgaagaaaca cacccagacg atgacagcta tattgtgcgt gtcaaggctg
      241 tggttatgac cagagatgac tccagcgggg gatggttccc acaggaagga ggcgggatca
      301 gtcgcgtcgg ggtctgtaag gtcatgcacc ccgaaggcaa tggacgaagc ggctttctca
      361 tccatggtga acgacagaaa gacaaactgg tggtattgga atgctatgta agaaaggact
      421 tggtctacac caaagccaat ccaacgtttc atcactggaa ggtcgataat aggaagtttg
      481 gacttacttt ccaaagccct gctgatgccc gagcctttga caggggagta aggaaagcaa
      541 tcgaagacct tatagaaggt tcaacaacgt catcttccac catccataat gaagctgagc
      601 ttggcgatga tgacgttttt acaacagcta cagacagttc ttctaattcc tctcagaaga
      661 gagagcaacc tactcggaca atctcctctc ccacatcctg tgagcaccgg aggatttata
      721 ccctgggcca cctccacgac tcatacccca cagaccacta tcacctcgat cagccgatgc
      781 caaggcccta ccgccaggtg agcttcccgg acgacgacga ggagatcgtg cgcatcaacc
      841 cccgggagaa gatctggatg acggggtacg aggattaccg gcacgcaccc gtcaggggca
      901 agtacccgga cccctcggag gacgcggact cctcctacgt gcgcttcgcc aagggcgagg
      961 tccccaagca tgactacaac tacccctacg tggactcctc agactttggc ctaggcgagg
     1021 accccaaagg ccgcgggggc agcgtgatca agacgcagcc ctcccggggc aagtcgcggc
     1081 ggcggaagga ggacggagag cgctcgcggt gcgtgtactg cagggacatg ttcaaccacg
     1141 aggagaaccg ccggggccac tgccaggacg cgcccgactc cgtgagaact tgcatccgcc
     1201 gggtgagctg catgtggtgc gcggacagca tgctctatca ctgtatgtcg gaccccgagg
     1261 gagactatac agacccttgc tcgtgcgata ctagcgacga gaagttttgc ctccggtgga
     1321 tggctcttat tgccttgtct ttcctggccc cctgtatgtg ctgttacctg ccccttcggg
     1381 cctgctacca ctgcggagtg atgtgcaggt gctgtggcgg gaagcacaaa gcggccgcgt
     1441 aatcaacttt cttgtacaaa gttggcatta taggaaagca ttgcttatca atttgttgca
     1501 acgaacaggt cactatcagt caaaataaaa tcattatttg catgcaagct tagatcctct
     1561 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     1621 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1681 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1741 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1801 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1861 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     1921 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1981 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2041 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2101 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2161 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2221 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2281 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2341 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2401 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2461 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2521 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2581 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2641 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2701 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2941 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3181 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3421 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3481 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3541 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3601 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3661 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     3721 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     3781 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     3841 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     3901 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
//
