LOCUS       sequence-126403-         819 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial 
            promoter. Cas genes are removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY002 (pFnCpf1_min)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        78..112
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 atcaagaggg caaaaaactc aatttggtta tcaaaaatga agagtatttt gagttcgtgc
       61 agaataggaa taactaattg acagctagct cagtcctagg tataatgcta gcgctgattt
      121 aggcaaaaac gggtctaaga actttaaata atttctactg ttgtagatga gaagtcattt
      181 aataaggcca ctgttaaaag tctaagaact ttaaataatt tctactgttg tagatgctac
      241 tattcctgtg ccttcagata attcagtcta agaactttaa ataatttcta ctgttgtaga
      301 tgtctagagc cttttgtatt agtagccggt ctaagaactt taaataattt ctactgttgt
      361 agattagcga tttatgaagg tcattttttt gtctagcttt aatgcggtag tttatcacag
      421 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
      481 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
      541 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
      601 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
      661 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
      721 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
      781 gccacaggtg cggttgctgg cgcctatatc gccgacatc
//
