LOCUS       sequence-126414-        1076 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS013
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(48..150)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     196..214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(260..278,279..284,285..301,302..307,308..332)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             342..1052
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(487..505)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     755..774
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
       61 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      121 tgaaagttgg aacctcttac gtgccgatca acggacgtct aagaaaccat tattatcatg
      181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagtcccta
      241 tcagtgatag agattgacat ccctatcagt gatagagatt gacatcccta tcagtgatag
      301 agatactgag cacatcagca ggacgcactg acaggtaccg catggtgagc aagggcgagg
      361 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggttcacatg gagggctccg
      421 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      481 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      541 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      601 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      661 gcggcgtggt gaccgtgacc caggactcct ccctgcaaga cggcgagttc atctacaagg
      721 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagactatgg
      781 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgcgctgaag ggcgagatca
      841 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      901 aggccaagaa gcccgtgcaa ctgcccggcg cgtacaacgt caacatcaag ttggacatca
      961 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1021 ccaccggcgg catggacgag ctgtacaagt aataatatcc gggtaggcgc aatcac
//
