LOCUS       sequence-126425-         883 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_omega2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    488..512
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     638..657
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     851..873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     865..882
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     866..882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggaacag
       61 aacttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
      121 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
      181 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
      241 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
      301 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
      361 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      421 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
      481 tggctggtgg caggatatat tgtggtgtaa acataacgat atcggtctca gtcaggagtg
      541 agacgcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      601 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
      661 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
      721 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggc gcaggcctct
      781 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      841 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtg
//
