LOCUS       sequence-126452-         987 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_alpha1
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..55
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    138..159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(174..179,180..197,198..204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    212..228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     217..239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     236..252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     236..252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    265..321
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(322..339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(322..338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(331..353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(547..566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    683..705
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 agatctcaaa caaacacata cagcgactta gtttacccgc caatatatcc tgtcagaatt
       61 cgtctcatga cagcgagaga ccccagctgg cacgacaggt ttcccgactg gaaagcgggc
      121 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      181 tatatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      241 aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
      301 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      421 cgaagaggcc tgcgccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      481 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      541 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      601 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtggg
      661 tctctctcct gagacgaatt cgttacacca caatatatcc tgccaagatc taattccggg
      721 gatcggaaat ccagaagccc gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa
      781 aaaaatttat aaaacgatct gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac
      841 ggtgggcaac cgcttgcggt tgtccacggg cggagccggt gcgcgtagcg cattgtccac
      901 aagccaaggg cgaccaataa ttgatatata tattcataat tgaaaagcta attgaacata
      961 ctacttgctg taactacttg ccggaac
//
