LOCUS       sequence-126540-        1003 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Jahreiss L, Menzies FM, Rubinsztein DC
  TITLE     The itinerary of autophagosomes: from peripheral formation to 
            kiss-and-run fusion with lysosomes.
  JOURNAL   Traffic. 2008 Apr;9(4):574-87. Epub 2008 Jan 7.
  PUBMED    18182013
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-04"; volume: "9(4)"; pages: "574-87. Epub 2008 Jan 7"
COMMENT     Sequence Label: mCherry-hLC3B-pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..788
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(226..244)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     494..513
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt acccgccacc atggtgagca agggcgagga ggataacatg gccatcatca
      121 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      241 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      301 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      421 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
      481 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      541 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
      601 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      661 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      721 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      781 tgtacaagca aggatccatg ccgtcggaga agaccttcaa gcagcgccgc accttcgaac
      841 aaagagtaga agatgtccga cttattcgag agcagcatcc aaccaaaatc ccggtgataa
      901 tagaacgata caagggtgag aagcagcttc ctgttctgga taaaacaaag ttccttgtac
      961 ctgaccatgt caacatgagt gagctcatca agataattag aag
//
