LOCUS       sequence-126695-         829 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Iwaki T, Figuera M, Ploplis VA, Castellino FJ
  TITLE     Rapid selection of Drosophila S2 cells with the puromycin resistance
            gene.
  JOURNAL   Biotechniques. 2003 Sep . 35(3):482-4, 486.
  PUBMED    14513552
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2003 Sep . 35(3):482-4, 486."
COMMENT     Sequence Label: pCoPURO
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    18..152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(42..61)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     96..115
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     167..183
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     168..184
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..420)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 attcgagctc ggtacccgat ccagacatga taagatacat tgatgagttt ggacaaacca
       61 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      121 ttgtaaccat tataagctgc aataaacaag ttcaagctag ctggcctcga ggtcgacggt
      181 atcgataagc ttgatctaga gtcggtgggc ctcgggggcg ggtgcggggt cggcggggcc
      241 gccccgggtg gcttcggtcg gagccatggg gtcgtgcgct cctttcggtc gggcgctgcg
      301 ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
      361 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
      421 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
      481 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
      541 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
      601 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
      661 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
      721 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
      781 cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggta
//
