LOCUS       sequence-12680-e         932 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: GFPZFN2-B2H
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             330..917
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ttaagcttgg taccgagctc ggatccacca tggactacaa agacgatgac gacccaaaaa
       61 agaagcgaaa ggtacccttc cagtgtcgca tttgcatgcg gaacttttcg gcgccgagca
      121 agcttgacag gcatacccgt actcatactg gtgaaaaacc gtttcagtgt cggatctgta
      181 tgcgaaattt ctccgatcgc tctaatctga cccgccatct acgtacgcac accggcgaga
      241 agccattcca atgccgaata tgcatgcgca acttcagtga gggggggaac ctgatgaggc
      301 acctaaaaac ccacacagga cagaaggacc aactagtcaa aagtgaactg gaggagaaga
      361 aatctgaact tcgtcataaa ttgaaatatg tgcctcatga atatattgaa ttaattgaaa
      421 ttgccagaaa ttccactcag gatagaattc ttgaaatgaa ggtaatggaa ttttttatga
      481 aagtttatgg atatagaggt aaacatttgg gtggatcaag gaaaccggac ggagcaattt
      541 atactgtcgg atctcctatt gattacggtg tgatcgtgga tactaaagct tatagcggag
      601 gttataatct gccaattggc caagcagatg aaatgcaacg atatgtcgaa gaaaatcaaa
      661 cacgaaacaa acatatcaac cctaatgaat ggtggaaagt ctatccatct tctgtaacgg
      721 aatttaagtt tttatttgtg agtggtcact ttaaaggaaa ctacaaagct cagcttacac
      781 gattaaatca tatcactaat tgtaatggag ctgttcttag tgtagaagag cttttaattg
      841 gtggagaaat gattaaagcc ggcacattaa ccttagagga agtgagacgg aaatttaata
      901 acggcgagat aaacttttaa tctagagggc cc
//
