LOCUS       sequence-127169-        1082 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  N-terminal tagging with mCitrine using URA3 as a selection marker in
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM42-mCitrine
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..65
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cttcgtacgc tgcaggtcga caacccttaa tataacttcg tataatgtat gctatacgaa
       61 gttattaggt ctagagatcc caatacaaca gatcacgtga tctttttgta agatgaagtt
      121 gaagtagtgt tgcaccgtgc caatgcaggt ggctattaga ttaaatatgt gatttgttct
      181 attaagtttc ctgtataatt aatggggagc gctgattctc ttttggtacg cttcccatcc
      241 agcatttctg tatctttcac cttcaacctt aggatctcta cccttggcga aaagtcctct
      301 gccaacaatg atgatatctg atccaccact tacaacttcg tcgacggttc tgtactgctg
      361 acccaatgca tcgcctttgt cgtctaaacc tacacctggg gtcatgatta gccaatcaaa
      421 cccttcttct cttcctccca tatcgttctg agcaatgaac ccaataacga aatctttatc
      481 actctttgca atatcaacgg tacccttagt atattcaccg tgtgctagag aacccttgga
      541 agacaattca gcaagcatca ataatcccct tggttctttg gtgacctctt gcgcaccttg
      601 tttcaagcca gcaacaatac cagcaccagt aaccccgtgg gcgttggtga tatcagacca
      661 ttctgcgata cggtaaacgc ccgatgtata ttgtaatttg actgtgttac cgatatcggc
      721 gaattttctg tcctcaaata tcaagaactt gtatttctct gccaatgctt tcaatggaac
      781 gacagtaccc tcataactga aatcatccaa gatatcaacg tgtgttttca aaaggcaaat
      841 gtatggaccc aacgtttcaa caagtttcaa tagctcatca gtcgaacgaa cgtcaagaga
      901 agcacacaaa ttggtcttct tttcatccat taaacgtaaa agtttcgatg caaccggact
      961 tgcatgagtc tcagctctac tggtatatga ttttgtggac atggtgcaac taattgacgg
     1021 gagtgtattg acgctggcgt actggctttc acaaaatggc ccaatcacaa ccacatctta
     1081 ga
//
