LOCUS       sequence-127568-         813 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-DHFRFLAG
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..81)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             578..600
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgtggtacc aagcttagat ctggatcttg tacattactt
       61 gtcatcatcg tccttgtagt cgccggcacg acgctcgagg atttcgaaac aatagctatg
      121 cgagttctgc gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc
      181 gtaatccgga aaatgggtgt cgccttccac ttctgcatcg atatgcgtca gatacagttt
      241 ttgcgctttt ggcaagaact gttcataaac gcgtccgccg ccaatcacca tgatttctgg
      301 tacgtcacca cacgccgcga tggcttcgtc gaccgattta acccaggtaa cccgatcatc
      361 ggtcccgggt tgactgctga ggataatatt tttgcggccg ggcaaaggcc taccgattga
      421 ttcccaggta tggcgcccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc
      481 gagatcggca ggcaggttcc atggcatggc gttttccatg ccgataacgc gatctaccgc
      541 tagcgccgca atcagactga tggatcccat atgtatatct ccttcttaaa gttaaacaaa
      601 attatttcta gagctagcga attccaattg gagctctccg gagccggagg gaaaccgttg
      661 tggtctccct atagtgagtc gtattaattt cgcgggatcg atgcatggtg ccaacaccta
      721 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
      781 tccataccgc cagttgttta ccctcacaac gtt
//
