LOCUS       sequence-127579-         619 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses mutated p53-L344A tagged with mKate2 and split C-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(74..95)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattatcaac tatgtataat aaagttgatt acttgtacag ctcgtccatg ccgagagtga
       61 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      121 tgctcagctt ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      181 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt
      241 ggcggatctt gaagttggcc ttgatgccgt tcttggctcc cccacctcca cctccacctc
      301 ctcctctgtg ccccagtttg ctagggaggt cgcagtatct ggccacagcc acctcgtgct
      361 gctcgacgta ggtctctttg tcggcctcct tgattctttc cagtcttctg tccacatagt
      421 agacgccggg catcttgagg ttcttagcgg gtttcttgga tctgtatgtg gtcttcaagt
      481 tgcagatcag gtggcccccg cccacgagct tcagggccat gtcggctctg ccttccaggc
      541 cgccgtcagc ggggtacagg gtctcggtgg aggcctccca gccgagtgtt ttcttctgca
      601 tcacagggcc gttggatgg
//
