LOCUS       sequence-127600-         602 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  TRC lentiviral vector for shRNA against mouse Dnd1 #7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLKO.1-shDnd1-No7
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(40..59)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(210..230)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(528..572)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
       61 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      121 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca
      181 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtgaagggg
      241 gcggccgctc gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg
      301 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
      361 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
      421 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
      481 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
      541 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
      601 aa
//
