LOCUS       sequence-127646-         983 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  CRISPR gRNA expression vector with an improved scaffold and puro/BFP
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    131..248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        323..571
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     323..343
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     494..513
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(733..756)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
      121 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      181 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      241 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ccgggcccta
      301 cgcgtccaag gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat
      361 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
      421 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      481 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      541 tttatatatc ttgtggaaag gacgaaacac cgggtcttcg agaagacacg tttaagagct
      601 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
      661 gcaccgagtc ggtgcttttt ttggatccaa ttctaccggg taggggaggc gcttttccca
      721 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      781 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      841 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      901 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      961 gacagcaccg ctgagcaatg gaa
//
