LOCUS       sequence-127654-         987 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with 
            an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S305A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     155..176
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
       61 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      121 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      181 ccgccgggat cactctcggc atggacgagc tgtacaagta ctcagatctc gagctcaagc
      241 ttggtaccat ggcgacctgc atcggggaga agatcgagga ttttaaagtt ggaaatctgc
      301 ttggtaaagg atcatttgct ggtgtctaca gagctgagtc cattcacact ggtttggaag
      361 ttgcaatcaa aatgatagat aagaaagcca tgtacaaagc aggaatggta cagagagtcc
      421 aaaatgaggt gaaaatacat tgccaattga aacatccttc tatcttggag ctttataact
      481 attttgaaga tagcaattat gtgtatctgg tattagaaat gtgccataat ggagaaatga
      541 acaggtatct aaagaataga gtgaaaccct tctcagaaaa tgaagctcga cacttcatgc
      601 accagatcat cacagggatg ttgtatcttc attctcatgg tatactacac cgggacctca
      661 cactttctaa cctcctactg actcgtaata tgaacatcaa gattgctgat tttgggctgg
      721 caactcaact gaaaatgcca catgaaaagc actatacatt atgtggaact cctaactaca
      781 tttcaccaga aattgccact cgaagtgcac atggccttga atctgatgtt tggtccctgg
      841 gctgtatgtt ttatacatta cttatcggga gaccaccctt cgacactgac acagtcaaga
      901 acacattaaa taaagtagta ttggcagatt atgaaatgcc atcttttttg tcaatagagg
      961 ccaaggacct tattcaccag ttacttc
//
