LOCUS       sequence-127798-         988 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2196
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..91
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     75..91
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    561..594
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa
       61 actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg
      121 ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat
      181 ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt
      241 gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat
      301 acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt
      361 ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat
      421 aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata
      481 taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg
      541 cccttattcg actcactata gaagttccta ttctctagaa agtataggaa cttcacttca
      601 ttttatttaa atttgcggcc gcgctgaggt cttaatgatc atagatccgg cacttagaga
      661 aagaccatag ttgaccaact tagattggct attgcgcttg ctatcgaagg caaccgataa
      721 caacggcaca cacagggccc caagtatgca cagcaccgac gatataagag aagacaacaa
      781 ccacctggga attttttcca ttttcttgcg tttcttcact gtgatgcttg tgagaatatt
      841 aggatatacc aacgtgcaac tctgaaatgt caaaacggtc gtgtataaat aaatgatata
      901 ccagtaggtg aaatcatctc atttgtcttt ctaaaaaagt ccattaccct taaggttgtt
      961 gtcacaaccc acggagcagt tttagagg
//
