LOCUS       sequence-127837-         987 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  co-expression of Cas9 and crRNA 589 target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP568-2
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..40
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        41..640
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 catgattacg ccaagctatc aactttgtat agaaaagttg gcacctttgg tcttttattg
       61 tcaacttcca ttggttcttc cattgtttct gttaaattaa tgaatttttc ataaaataaa
      121 gacattatac aatataaaaa tgaagaattt attgaaaata aactgccaga gagaaaaagt
      181 atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg tacattttcg tgctaggagt
      241 tagatgtgca ggcagcaacg agagggggag agattttttt gggccttgtg aaattaacgt
      301 gagttttctg gtcatctgac taatcatgtt ggttttttgt tggtttattt tgtttttatc
      361 tttgttttta tccagattag gaaatttaaa ttttatgaat ttataatgag gtcaaacatt
      421 cagtcccagc gtttttcctg ttctcactgt ttagtcgaat ttttatttta ggctttcaac
      481 aaatgttcta actgtcttat ttgtgacctc actttttata tttttttaat ttttaaaaat
      541 attagaagtt tctaggataa ttttttcgac ttttattctc tctaccgtcc gcactcttct
      601 tacttttaaa ttaaattgtt tttttttcag ttgggaaaca ctttgctcta gaaaaaatgg
      661 acaaaaaata cagcatcggc cttgacatcg gaacgaactc cgtcggatgg gccgtcatca
      721 ccgacgagta caaggtccca tccaagaagt tcaaggtcct cggaaacacc gaccgtcact
      781 ccatcaagaa gaacctcatc ggagccctcc tcttcgactc cggagagacc gccgaggcca
      841 cccgtctcaa gcgtaccgcc cgtcgtcgtt acacccgtcg taagaaccgt atctgctacc
      901 tccaagagat cttctccaac gagatggcca aggtcgacga ctccttcttc caccgtctcg
      961 aggagtcctt cctcgtcgag gaggaca
//
