LOCUS       sequence-127845-         793 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  co-expression of Cas9 and crRNA 589 target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP568-2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(57..79)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(351..426)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(447..793)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
ORIGIN
        1 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
      301 gggcgatcgg tgcgggcctc ttcgctatta cgccagaaat ttcacaaaaa gcaccgactc
      361 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
      421 taaaacctcg tggtgcctat ggtagcaaga catctcgcaa taggaggtgt ttatataggg
      481 gcaacacaag aggcggcaga catctcgtga gtttgacaga gtagtataaa gagtgtgtgt
      541 gagagggtgt aggcatttca taacatattt tttgtggaat aattttggaa aaaaggtgtt
      601 tttttaataa tagcttttat aagagtttct caaaaattgc atgctttttt tgaaaaggtt
      661 gacaaattat agtagtacta aagttgctat tttcaggtct cagcagaaaa ttcacaacta
      721 aaaataaaat ttgcaaaaca gagtgtgcgc ctttaaggtg tacagttttt tattccttta
      781 ttgctagttt ttt
//
