LOCUS       sequence-127886-        1048 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses TEV::eGFP::myc::3Xflag in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP682-1
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..117)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(169..198)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(211..921,922..924))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(251..271)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     866..894
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(925..945)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(972..989)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(972..988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(981..1003)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccttgtcatc
       61 gtcatccttg taatcgatat catgatcttt ataatcaccg tcatggtctt tgtagtcact
      121 agttctagag cggccgccac cggtggagct ccaccgatcc cccgggcaca gatcctcttc
      181 tgatatcagt ttttgttcgc cggctacgta cttgtacagc tcgtccatgc cgagtgtaat
      241 cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt
      301 cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc
      361 aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg
      421 tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt
      481 gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc
      541 aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg
      601 tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg
      661 catggcactc ttgaaaaagt catgctgctt catgtggtcg gggtaccggc tgaagcactg
      721 cacgccgtag gtcagagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt
      781 gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac
      841 agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga caactccagt
      901 gaaaagttct tctcctttac tcactccttg gaagtagagg ttctcgatcc ccgggtaccg
      961 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1021 caacttaatc gccttgcagc acatcccc
//
