LOCUS       sequence-128090-        7097 bp    DNA     circular SYN 14-APR-2025
DEFINITION  TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO tTR-KRAB-rtTA3-Luc
FEATURES             Location/Qualifiers
     source          1..7097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             332..952
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(405..424)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             953..973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1076..1270
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             1352..1408
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1409..2113
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     CDS             2129..2185
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2186..3838
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2252..2270)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(3984..4003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3985..4003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4010..4027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4010..4026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4019..4041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4164..4466
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4345..4364
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             4815..5609
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4815..5609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4869..4888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5479..5498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      5810..5819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5816..6190
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      6328..6916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6817..6836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7070..7087
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcct
      301 aactcgaacg ctagctgtgc gatcgtttac catggctaga ttagataaaa gtaaagtgat
      361 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
      421 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
      481 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
      541 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
      601 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
      661 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
      721 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
      781 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
      841 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
      901 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cgccaaaaaa
      961 gaagagaaag gtcgacggcg gtggtgcttt gtctcctcag cactctgctg tcactcaagg
     1021 aagtatcatc aagaacaagg agggcatgga tgctaagtca ctaactgcct ggtcccggac
     1081 actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga agctgctgga
     1141 cactgctcaa caaatcgtgt acagaaatgt gatgctggag aactataaga acctggtttc
     1201 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     1261 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     1321 aatcaaatca tcagttaaac tgggcagcgg tgccactaac ttcagcttgt tgaagcaggc
     1381 cggagacgtc gaagagaacc cgggtccaat gtctagactg gacaagagca aagtcataaa
     1441 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     1501 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     1561 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     1621 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     1681 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     1741 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     1801 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     1861 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     1921 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     1981 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     2041 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgatt ttgaccttga
     2101 catgctcccc gggaagttag gatccggagc cacgaacttc tctctgttaa agcaagcagg
     2161 agacgtggaa gaaaaccccg ggcccatgga agacgccaaa aacataaaga aaggcccggc
     2221 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     2281 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
     2341 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     2401 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     2461 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     2521 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
     2581 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
     2641 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     2701 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
     2761 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     2821 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     2881 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     2941 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     3001 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt
     3061 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     3121 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca
     3181 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac
     3241 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     3301 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt
     3361 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     3421 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     3481 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc
     3541 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct
     3601 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     3661 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     3721 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     3781 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaata
     3841 aaaacgttgt tgtttggggt tgaattactc tggatccact agtaacggcc gccagtgtgc
     3901 tggaattcgc ccttaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc
     3961 atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     4021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4141 agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     4201 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     4261 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4321 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4381 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4441 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     4501 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     4561 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4621 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4681 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4741 ctttctcgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     4801 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4861 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4921 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4981 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5041 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5101 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5161 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5221 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5281 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5341 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5401 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5461 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5521 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5581 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     5641 attttctcct tacgcatctg tgcggtattt cacaccgcat acaggtggca cttttcgggg
     5701 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5761 catgagacaa taaccctgat aaatgcttca ataatagcac gtgaggaggg ccaccatggc
     5821 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     5881 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     5941 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     6001 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     6061 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     6121 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     6181 gcaggactga cacgtgctaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6241 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg gcttttgctg
     6961 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7021 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7081 gagcgaggaa gcggaag
//
