LOCUS       sequence-128328-         734 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses wild type zebrafish Chk1 with C-term GFP fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Zhang M, Kothari P, Mullins M, Lampson MA
  TITLE     Regulation of zygotic genome activation and DNA damage checkpoint 
            acquisition at the mid-blastula transition.
  JOURNAL   Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066.
  PUBMED    25558827
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2014"; volume: "13(24):3828-38. doi"; pages: " 
            10.4161/15384101.2014.967066"
COMMENT     Sequence Label: pMZ030
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caggaccatg gctgtgcctt
      121 ttgttaaaga ctgggatgtg gtacaaactc ttggagaggg agcatatgga gaggtgcgac
      181 tgctggtcaa caagaaaaca gaagaggctg tggcggtgaa agttgtggac atggcaaaag
      241 ccaaggattg catcgagaat gtgaagaagg aggtctgcat atgcaagatg ctttcacacc
      301 ccaacattgt acgtttcttt gggcacagtg ttgggattac acatcgtgac ataaagcctg
      361 agaatattct tcttgatgat aaagataatc tgaagatctc tgactttggc ctggctacca
      421 tgttcaggca ccgtggccgt gagcgagctt tgaaccgtct gtgtggtact ctgccctatg
      481 ttgccccaga gttgatgtca cgctcatctt ttaacgctca gcctgcggac acttgggctt
      541 gtggcattgt gctcactgca atgttagctg gagagttacc ctgggatcag ccgagtgaaa
      601 actgtcagga atatttggac tggctggaaa gaaagaccta ccttacaccc tggaagaaaa
      661 ttgatgcggt accccttagt ctgttgtcta agatattact gcacaatcca gaagacaggt
      721 tcaccattcc tgaa
//
