LOCUS       sequence-12845-e       11811 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Yudushkin IA, Vale RD.
  TITLE     Imaging T-cell receptor activation reveals accumulation of 
            tyrosine-phosphorylated CD3{zeta} in the endosomal compartment.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22128-33.
  PUBMED    21135224
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-12-21"; volume: "107(51)"; pages: 
            "22128-33"
COMMENT     Sequence Label: pHR-ZIP(RK)
FEATURES             Location/Qualifiers
     source          1..11811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1027..1049
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1087..1105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1173..1277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1278..1346,1347..2138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1496..1515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2309..2897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2798..2817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3051..3068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3138..3158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3143..3472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3323..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4606..4671
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4801..4821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5246..5380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5283..5302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5337..5356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5549..6182
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6229..6354
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6851..7084
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7269..7313
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7598..7715
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7868..8275
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8816..8825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8822..8824,8825..8827,8828..9538)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8822..8824,8825..8827,8828..9538)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8867..8888)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9128..9147)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9475..9496
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9590..10297
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9735..9753)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10003..10022
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    10313..10337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11141..11158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    11221..11809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11274..11294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11692..11703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     6721 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6781 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6841 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6901 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6961 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7021 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7081 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7141 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7201 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7261 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7321 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7381 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7441 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     7501 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     7561 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     7621 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     7681 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7741 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
     7801 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     7861 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
     7921 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
     7981 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     8041 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     8101 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     8161 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     8221 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     8281 tctggagctc tcgagaattc tcacgcgtgc caccatgaag tggaaagtgt ctgttctcgc
     8341 ctgcatcctc cacgtgcggt tcccaggagc agaggcacag agctttggtc tgctggatcc
     8401 caaactctgc tacttgctag atggaatcct cttcatctac ggagtcatca tcacagccct
     8461 gtacctgaga gcaaaattca gcaggagtgc agagactgct gccaacctgc aggaccccaa
     8521 ccagctctac aatgagctca atctagggcg aagagaggaa tatgacgtct tggagaagaa
     8581 gcgggctcgg gatccagaga tgggaggcaa acagcagagg aggaggaacc cccaggaagg
     8641 cgtatacaat gcactgcaga aagacaagat ggcagaagcc tacagtgaga tcggcacaaa
     8701 aggcgagagg cggagaggca aggggcacga tggcctttac cagggtctca gcactgccac
     8761 caaggacacc tatgatgccc tgcatatgca gaccctggcc cctcgcccac cggtcgccac
     8821 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     8881 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     8941 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     9001 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     9061 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     9121 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     9181 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     9241 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     9301 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     9361 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     9421 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     9481 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     9541 accaggatcg gcgtctgggg aaggactgcc aggatcagcc ggaccaggaa tggtgagcaa
     9601 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     9661 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     9721 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     9781 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     9841 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     9901 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     9961 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
    10021 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
    10081 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
    10141 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
    10201 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
    10261 ccgccactcc accggcggca tggacgagct gtacaagcga tcgcaccggt atacaagttt
    10321 gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg ccagaccccg cggcgcacct
    10381 gcccttcttc tacggcagca tctcgcgtgc cgaggccgag gagcacctga agctggcggg
    10441 catggcggac gggctcttcc tgctgaagca gtgcctgcgc tcgctgggcg gctatgtgct
    10501 gtcgctcgtg cacgatgtgc gcttccacca ctttcccatc gagcgccagc tcaacggcac
    10561 ctacgccatt gccggcggca aagcgcactg tggaccggca gagctctgcg agttctactc
    10621 gcgcgacccc gacgggctgc cctgcaacct gcgcaagccg tgcaaccggc cgtcgggcct
    10681 cgagccgcag ccgggggtct tcgactgcct gcgagacgcc atggtgcgtg actacgtgcg
    10741 ccagacgtgg aagctggagg gcgaggccct ggagcaggcc atcatcagcc aggccccgca
    10801 ggtggagaag ctcattgcta cgacggccca cgagcggatg ccctggtacc acagcagcct
    10861 gacgcgtgag gaggccgagc gcaaacttta ctctggggcg cagaccgacg gcaagttcct
    10921 gctgaagccg cggaaggagc agggcacata cgccctgtcc ctcatctatg ggaagacggt
    10981 gtaccactac ctcatcagcc aagacaaggc gggcaagtac tgcattcccg agggcaccaa
    11041 gtttgacacg ctctggcagc tggtggagta tctgaagctg aaggcggacg ggctcatcta
    11101 ctgcctgaag gaggcctgcc ccaacagcag tgccccaggg caccatcatc atcatcatta
    11161 agcggccgcg actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat
    11221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    11281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    11341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    11401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    11461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    11521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    11581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    11641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    11761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca t
//
