LOCUS       sequence-128785-         825 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Constiutive mammalian expression of the bioluminescent OgNLuc 
            LumiFluor reporter for single-cell analysis and sensitive optical 
            imaging of living subjects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, 
            Cleveland JL, Amelio AL
  TITLE     Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical 
            Imaging and Flow Cytometry for Monitoring Tumorigenesis.
  JOURNAL   Cancer Res. 2015 Sep 30. pii: canres.3538.2014.
  PUBMED    26424696
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Sep 30. pii: canres.3538.2014."
COMMENT     Sequence Label: pRetroX-Tight-MCS_PGK-OgNLuc
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..731
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     437..456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggcctttcga cctgcagccc aagatggtga gcaagggcga ggagaataac atggccatca
       61 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
      121 agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgtt aagctgaagg
      181 tgaccaaggg tggccccctg cccttcgcct gggacatctt gtcccctcag ttcacctacg
      241 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacctcaag ctgtccttcc
      301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      361 ctcaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      421 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggcatggag gcctcctccg
      481 agcggatgta ccccgaggac ggcgccctga agggcgagga caagctcagg ctgaagctga
      541 aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag aagcccgtgc
      601 agttgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac aacgaggact
      661 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      721 agctgtacaa ggatatctcc ggaggtatgg tcttcacact cgaagatttc gttggggact
      781 ggcgacagac agccggctac aacctggacc aagtccttga acagg
//
