LOCUS       sequence-128921-         698 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Cas9 with fluorescent Cherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FUCas9Cherry
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..85)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 caccgcatgt tagcagactt cctctgccct caccgccctt tttctttttt gcctggccgg
       61 cctttttcgt ggccgccggc cttttgtcgc ctcccagctg agacaggtcg atccgtgtct
      121 cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct ttggtgctgg
      181 tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat
      241 tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc ttatcccggt
      301 gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc actctcttgg
      361 agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca
      421 gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg ctggccaggt
      481 acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc agttcgccgg
      541 cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac ttaggcagct
      601 tgatgatcag gtcctttttc acttctttgt agcccttggc ttccagaaag tcgatgggat
      661 tcttctcgaa gctgcttctt tccatgatgg tgatcccc
//
