LOCUS       sequence-128947-         394 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB404
FEATURES             Location/Qualifiers
     source          1..394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(74..80,81..98,99..104))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(302..319)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(302..318)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(311..333)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga cggatcctaa
       61 ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      121 gcggcccctc gaggggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtttgat tagagtcgac ctgcaggcat gcaagcttgg
      301 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      361 gccttgcagc acatccccct ttcgccagct ggcg
//
